EXPERT IN BIOCHIPS DESIGN AND DEVELOPMENT
We carry out biochips from the probes design to the data analysis and we provide services to perform tests with your samples.
We have a patented technology to functionalize the support allowing the maximized grip of the probes in a “3D” environment. This technology optimizes the signal-to-noise ratio resulting from the hybridization of the probes, which greatly increases the DendrisChip sensitivity.
We can realize your customized biochips after defining together their contents, the biological context, the type of probes and the amount of biochips to produce.
Oligonucleotide probes, if not defined by yourself, can be selected by our data analyst, by bioinformatics methods within public databases to allow identification of organisms sought during the diagnosis.
The spotter robot used for the deposition of oligonucleotides on glass slides is Scienion’s “sciFlexarrayer SX”.
The inkjet technique used allows to deposit very small quantities of solutions thanks to electric piezos (of the order of Pico and nanolitre) to form a spot on the slide surface with an accuracy less than 3 μm. The spotter robot can achieve 130 slides per run.
Slides we use have unique identification. In routine, Slides are spotted with the identification at the bottom of the slide.
The robot includes a control quality system.
Each run is validated only if 100% of spots are compliant.
Our expertise focuses not only on biochips technology but also on data analysis. Dendris has developed a method of interpreting signals according to the principles of “machine learning”. Thus Dendris products are able to take into account evolutions of the reference data and constantly adapt to the analyzed context.
DendrisChips and data acquisition are performed using the DendriScan scanner associated with the MAPIX software (Innopsys). The DendriScan has a 635nm laser that excites Cyanine 5. The fluorescence emitted at the emission wavelength of the excited fluorochrome is captured by a photomultiplier present in the scanner. The resolution is set at 10 μm per pixel. The resulting TIFF image is processed to extract the specific data for each spot. A reading grid is applied to the image to delimit spots thanks to an automatic search by the software. A digital transformation step is then performed by the software in order to obtain in a single .GPR file the data relating to each spot.
Signals emitted by the biochip are interpreted by the Dendrisoftdiag, an artificial intelligence (machine learning) software designed by Dendris, which relies on a database constantly enhanced by new samples.
In order to best meet your request, we define together the content of the experiments to be carried out.
At the end of our discussion, we will provide you with a commercial proposal. All steps will be discussed together.
In 2019, Dendris signs a partnership with LALLEMAND / UMR for the development of a biochip for diagnosis of ruminal fibrolytic activity.
Lallemand, world leader in the development, production and sale of yeasts, bacteria and derived ingredients, associated with UMR MEDIS (Digestive environment and health microbiology dependent on Inra and university) asked Dendris to develop a biochip for studying the intestinal microbiota in animals.
This program will be based on the work carried out by LALLEMAND / UMR MEDIS on the identification of biomarkers characteristic of ruminal fibrolytic activity. The aim is to verify the application of Dendris technology in this context.