EXPERT IN BIOCHIPS DESIGN AND DEVELOPMENT
We carry out biochips from the probes design to the data analysis and we provide services to perform tests with your samples.
We have a patented technology to functionalize the support allowing the maximized grip of the probes in a “3D” environment. This technology optimizes the signal-to-noise ratio resulting from the hybridization of the probes, which greatly increases the DendrisChip sensitivity.
From design to analysis
Probes design
We can realize your customized biochips after defining together their contents, the biological context, the type of probes and the amount of biochips to produce.
Oligonucleotide probes, if not defined by yourself, can be selected by our data analyst, by bioinformatics methods within public databases to allow identification of organisms sought during the diagnosis.
Data analysis
Our expertise focuses not only on biochips technology but also on data analysis. Dendris has developed a method of interpreting signals using machine learning. Thus Dendris products are able to take into account evolutions of the reference data and constantly adapt to the analyzed context.
DendrisCHIPS reading and data acquisition are performed using the DendriSCAN scanner associated with the sciREADER software (Scienion) allowing the reading of several slides simultaneously. The DendriSCAN applies a reading grid on the image to determine spots intensity thanks to an automatic search by the software. The resolution is fixed at 5 μm per pixel. All of the data is then saved in a single excel file.
The resulting file is interpreted by DendriSOFT, Artificial Intelligence software designed by Dendris, which relies on a database constantly enriched by new samples.
Spotting
The robot is able to dispense really small liquids quantities (300 pL) using the piezoelectric effect. It results with a spot on the target surface made with a precision over 3 µm. We can produce 140 slides or 24 SBS plates, which represent up to 2000 tests per run depending of the number of designed probes.
The quality control takes a great place in this process with two important steps. Firstly, the detection of the presence and aspect of the droplet both before and after the dispensing on the surface. Secondly, pictures of the spotted zone control the presence of the spot on the surface.
sciFLEXARRAYER SX (Scienion) used for probes dispensing
Project exemple
In 2019, Dendris signs a partnership with LALLEMAND / UMR for the development of a biochip for diagnosis of ruminal fibrolytic activity.
Lallemand, world leader in the development, production and sale of yeasts, bacteria and derived ingredients, associated with UMR MEDIS (Digestive environment and health microbiology dependent on Inra and university) asked Dendris to develop a biochip for studying the intestinal microbiota in animals.
This program will be based on the work carried out by LALLEMAND / UMR MEDIS on the identification of biomarkers characteristic of ruminal fibrolytic activity. The aim is to verify the application of Dendris technology in this context.